Shinuo Cao, Heming Chen, Xiaohan Liang, Jiawen Fu, Shida Wang, Jun Zheng, Zhaoxia Zhang, Yu Pang, Jingfei Wang, Bang Shen, Honglin Jia

Mol Microbiol. 2019 Oct 31. doi: 10.1111/mmi.14411

Abstract

Post-Golgi vesicle trafficking is indispensable for precise movement of proteins to the pellicle, the sub-pellicle network, and apical secretory organelles in Apicomplexa. However, only a small number of molecular complexes involved in trafficking, tethering, and fusion of vesicles have been identified in Toxoplasma gondii. Consequently, it is unclear how complicated vesicle trafficking is accomplished in this parasite. Sec1/Munc18-like (SM) proteins are essential components of protein complexes involved in vesicle fusion. Here, we found that depletion of the SM protein TgSec1 using an auxin-inducible degron-based conditional knockout strategy led to mislocalization of plasma membrane proteins. By contrast, conditional depletion of the SM protein TgVps45 led to morphological changes, asymmetrical loss of the inner membrane complex and defects in nucleation of sub-pellicular microtubules, polarization, and symmetrical assembly of daughter parasites during repeated endodyogeny. TgVps45 interacts with the SNARE protein TgStx16 and TgVAMP4-1. Conditional ablation of TgStx16 causes the similar growth defect like TgVps45 deficiency suggested they work together for the vesicle fusion at TGN. These findings indicate that these two SM proteins are crucial for assembly of pellicle and sub-pellicle network in T. gondii, respectively.

© 2019 John Wiley & Sons Ltd.

KEYWORDS:

Toxoplasma gondii ; Sec1; Sec1/Munc18-like protein; Stx16; Vps45