Ping Chen,Ran Liu, Mengmeng Huang, Jinlu Zhu, Dong Wei, Francis J Castellino, Guanghui Dang, Fang Xie, Gang Li, Ziyin Cui, Siguo Liu, Yueling Zhang

J Biol Chem . 2020 Jun 9;jbc.RA119.011977.doi: 10.1074/jbc.RA119.011977. Online ahead of print.

Abstract

Infections by many bacterial pathogens rely on their ability to degrade host glycans by producing glycoside hydrolases (GHs). Here, we discovered a conserved multifunctional GH, SsGalNagA, containing a unique combination of two family 32 carbohydrate-binding modules (CBM), a GH16 domain and a GH20 domain, in the zoonotic pathogen Streptococcus suis 05ZYH33. Enzymatic assays revealed that the SsCBM-GH16 domain displays endo-(β1,4)-galactosidase activity specifically toward the host-derived αGal epitope Gal(α1,3)Gal(β1,4)Glc(NAc)-R, whereas the SsGH20 domain has a wide spectrum of exo-β-N-acetylhexosaminidase activities, including exo-(β1,3)-N-acetylglucosaminidase activity, and employs this activity to act in tandem with SsCBM-GH16 on the αGal-epitope glycan. Further, we found that the CBM32 domain adjacent to the SsGH16 domain is indispensable for SsGH16 catalytic activity. Surface plasmon resonance experiments uncovered that both CBM32 domains specifically bind to αGal-epitope glycan, and together they had a KD of 3.5 mM toward a pentasaccharide αGal-epitope glycan. Cell-binding and αGal epitope-removal assays revealed that SsGalNagA efficiently binds to both swine erythrocytes and tracheal epithelial cells and removes the αGal epitope from these cells, suggesting that SsGalNagA potentially functions in nutrient acquisition or alters host signaling in S. suis. Both binding and removal activities were blocked by an αGal-epitope glycan. SsGalNagA is the first enzyme reported to sequentially act on a glycan containing the αGal epitope. These findings shed detailed light on the evolution of GHs and an important host-pathogen interaction.

Keywords: GalNagA; Streptococcus; carbohydrate processing; carbohydrate-binding protein; endo-(β1,4)-galactosidase; enzyme catalysis; enzyme kinetics; erythrocyte; glycoside hydrolase; host-pathogen interaction; multifunctional enzyme; substrate specificity; αGal epitope; β-acetylhexosaminidase.

Published under license by The American Society for Biochemistry and Molecular Biology, Inc.